首页> 外国专利> Process for developing a baker's yeast strain strain of saccharomyces cerevisiae from industrial baker's yeast its use process for preparing a chilled and packaged baker's dough and obtained baker's dough

Process for developing a baker's yeast strain strain of saccharomyces cerevisiae from industrial baker's yeast its use process for preparing a chilled and packaged baker's dough and obtained baker's dough

机译:由工业面包酵母制备啤酒酵母的酿酒酵母菌株的方法及其用于制备冷藏包装的面包面团并获得面包面团的使用方法

摘要

Process for producing a source of baker's yeast exhibiting a Lti (Low Temp. Inactive) phenotype, i.e. the property of being practically inactive but surviving in a dough at a temp. 14 degrees C at which the dough does not freeze, and of being practically inactive at 18 degrees C in a maltose-contg. medium, in which firstly a strain of haploidal Saccharomyces cerevisiae that is inactive at a temp. of 3-10 degrees C, pref. 10 degrees C, is crossed with a strain of haploidal Saccharomyces cerevisiae having 1 MAL allele that is active but with catabolic repression. In a second stage, the segregants are crossed and finally a diploidal prototrophic strain is selected exhibiting a Lti phenotype, an active MAL phenotype that is active but with catabolic suppression, and a potential to be grown in a fed-batch process. Also claimed are Saccharomyces cerevisiae NCIMB 40612 and NCIMB 40611.
机译:生产表现出Lti(低温无活性)表型的面包酵母来源的方法,即实际上无活性但可以在面团中在一定温度下存活的特性。面团在14摄氏度时不冻结,在含有麦芽糖的18摄氏度时几乎没有活性。培养基,其中首先是在一定时间内没有活性的单倍体酿酒酵母菌株。 3-10摄氏度,优选。 > 10℃,与具有1个MAL等位基因的单倍体酿酒酵母菌株杂交,该菌株具有活性但具有分解代谢抑制作用。在第二阶段中,使分离物杂交,最后选择二倍体原养型菌株,其表现出Lti表型,具有活性但具有分解代谢抑制作用的活性MAL表型以及在分批补料过程中生长的潜力。还要求保护的是酿酒酵母NCIMB 40612和NCIMB 40611。

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