首页>
外国专利>
METHOD FOR SIMULTANEOUSLY DETECTING SYNTHESIZED AMOUNT OF REPAIR DNA OF CELL AND SYNTHESIZED AMOUNT OF REPLICATIVE DNA
METHOD FOR SIMULTANEOUSLY DETECTING SYNTHESIZED AMOUNT OF REPAIR DNA OF CELL AND SYNTHESIZED AMOUNT OF REPLICATIVE DNA
展开▼
机译：同时检测细胞修复DNA合成量和复制DNA合成量的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
PURPOSE: To provide a method for simultaneously detecting the synthesized amount of repair DNA of a cell and the synthesized amount of replicative DNA, capable of being simply and rapidly carried out and measuring carcinogenicity of a chemical substance. ;CONSTITUTION: A first group in which a hepatocyte is floated in a culture solution and the number of cells is adjusted is mixed with a culture solution not containing hydroxyurea (HU) and radioisotope (RI) and cultured. A second group is mixed with a HU-containing culture solution and RI and cultured. A third group is mixed with the culture solution not containing HU, RI and a chemical substance specimen and cultured. A fourth group is the HU-containing culture solution, RI and the chemical substance specimen. After the culture, the culture solution is removed, RI not taken in a deoxyribonucleic acid (DNA) is removed. Then the DNA is made into a naked nucleus and purified. The total synthesized amount of each DNA-protein complex is measured for the first to fourth DNA-protein complexes derived from the first to fourth groups obtained by the DNA purification to calculate simultaneous equations formed between the DNA-protein complexes.;COPYRIGHT: (C)1995,JPO
展开▼
