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D-arabinitol dehydrogenase from Candida tropicalis ATCC 750 or Candida shehatae
D-arabinitol dehydrogenase from Candida tropicalis ATCC 750 or Candida shehatae
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机译：热带假丝酵母ATCC 750或shehatae的D-阿拉伯糖醇脱氢酶
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摘要
A D-arabinitol dehydrogenase enzyme is disclosed. The enzyme is capable of catalyzing the oxidation of D-arabinitol and substantially incapable of catalyzing the oxidation of D-mannitol and is substantially free of other enzymes capable of oxidizing D-mannitol. Also disclosed are methods for determining D-arabinitol. In one embodiment the method comprises the steps of providing in combination (1) a medium suspected of containing D-arabinitol and (2) a D-arabinitol dehydrogenase enzyme and examining the medium for the product of the oxidation of the D-arabinitol. The enzyme utilized is capable of catalyzing the oxidation of D- arabinitol and substantially incapable of catalyzing the oxidation of D- mannitol. Kits for conducting the present method are also disclosed. The D-arabinitol dehydrogenase in purified form has all of the characteristics of a D-arabinitol dehydrogenase which is obtainable from Candida tropicalis ATCC 750 or Candida shehatae.
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