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Procedure for the determination of mutations in the DNA of a genetically engineered mammal or a genetically engineered mammalian cell.
Procedure for the determination of mutations in the DNA of a genetically engineered mammal or a genetically engineered mammalian cell.
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机译:测定基因工程哺乳动物或基因工程哺乳动物细胞的DNA突变的方法。
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PCT No. PCT/NL92/00062 Sec. 371 Date Dec. 29, 1993 Sec. 102(e) Date Dec. 29, 1993 PCT Filed Apr. 2, 1992 PCT Pub. No. WO92/17605 PCT Pub. Date Oct. 15, 1992Detection of mutations in a lacZ gene including lacZ operator sequence functioning as marker gene located in a plasmid whereof one or more copies are introduced into the DNA of a mammal or of a mammalian cell, by isolating the DNA, cutting the plasmid out of the DNA, picking up the plasmid DNA with solid particles to which a lacZ operator binding material is bound, again releasing the plasmid from the solid particles after isolation and subsequently circularizing the plasmid, transforming a host restriction-negative, lacZ-negative and galE-negative bacterial host with the plasmid and separating transformants with mutated lacZ gene which grow on a lactose-containing medium from transformants with a non-mutated lacZ gene which do not grow on such a medium. A transgenic mammal model for mutation analysis.
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