首页> 外国专利> method oligonukleotidprimers and oligonukleotidsonden for the detection of pathogenic bacteria in the oral cavity.

method oligonukleotidprimers and oligonukleotidsonden for the detection of pathogenic bacteria in the oral cavity.

机译:寡核苷酸的引物和寡核苷酸的检测方法,用于检测口腔中的致病菌。

摘要

Development of highly sensitive processes for the detection of anaerobic bacteria and microaerophilic pathogens of the buccal cavity from samples taken directly by the practitioner. Current research in odontology shows that anaerobic bacteria or gram-negative micro-aerophils play an important role in diseases of the buccal cavity, especially those affecting the paradentium. Research into these pathogens is important for the practitioner to make a precise diagnosis and adapt the therapeutic treatment. The invention disclosed herein consists of a process for taking and processing samples, amplifying the DNA contained in the latter by using an oligonucleotide primers for amplification of the DNA of the pathogens, followed by hybridization of the amplification product with an oligonucleotide probe specific to pathogens whose presence is to be detected.
机译:开发高灵敏度的方法,用于从从业者直接获取的样品中检测口腔中的厌氧细菌和微需氧菌病原体。目前在牙科学上的研究表明,厌氧细菌或革兰氏阴性微需氧菌在颊腔疾病,尤其是影响牙周炎的疾病中起重要作用。对这些病原体的研究对于从业人员进行准确的诊断和调整治疗方法很重要。本文公开的本发明包括以下过程:获取和处理样品,通过使用寡核苷酸引物扩增病原体的DNA,扩增样品中包含的DNA,然后将扩增产物与对病原体特异的寡核苷酸探针杂交。存在将被检测到。

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