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首页> 外国专利> PROCESS FOR MANUFACTURING INACTIVATED VACCINE USED AGAINST VIRAL HEMORRHAGIC DISEASES OF RABBITS

PROCESS FOR MANUFACTURING INACTIVATED VACCINE USED AGAINST VIRAL HEMORRHAGIC DISEASES OF RABBITS

机译：制造用于治疗兔病毒性血液病的灭活疫苗的方法

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FIELD: bioengineering; veterinary. SUBSTANCE: essence of this method resides in using 20 to 25-percent suspension of livers of rabbits died from infection caused by virulent hemorrhagic disease (buffered physiologic salt solution, pH value: 7.2 to 7.4), inactivating virus stock by using 1,8,3,6-diendomethylene-1,3,6,8-tetraazotricyclodecane (A-24) at rate of 1 g of preparation per 1000 ml of stock, using 1 part of chloroform per 5 parts of virus-containing suspension for freeing stock of cellular detritus, stabilizing stock with use of 5-percent mixture of lactose and peptone dissolved in buffer solution (pH value: 7.2 to 7.4), taken in equal proportions and admixed with inactivated suspension of liver (1:2), carrying out lyophilization after freezing at temperature of minus 45 to 50 C during 10 to 12 h, standing stock at temperature of minus 45 to 0 C during 22 to 24 h and vacuum of 11.0 to 15.0 Pascals, bringing temperature to point of 18 to 20 C during 3 to 4 h, and drying resultant material during 2 to 3 h. EFFECT: more sophisticated technique. 3 tbl
机译：领域：生物工程;兽医。实质：该方法的本质在于使用由剧烈出血性疾病（缓冲生理盐溶液，pH值：7.2至7.4）引起的感染死亡的家兔肝脏悬浮液的20％到25％，使用1,8灭活病毒原种， 3,6-diendomethyl-1,3,6,8-四偶氮三环癸烷（A-24）以每1000 ml储备液1 g制剂的速率使用，每5份含病毒悬浮液使用1份氯仿，以释放出细胞碎屑，使用溶于缓冲液（pH值：7.2至7.4）中的5％的乳糖和蛋白mixture混合物稳定匀浆，按等比例混合并与灭活的肝悬浮液（1：2）混合，在冻干后进行冻干在10到12个小时内在负45至50摄氏度的温度下冷冻，在22到24个小时内在负45至0摄氏度的温度下静置，在11.0到15.0帕斯卡的真空下，在3至7摄氏度将温度升高到18到20摄氏度4小时，并在2-3小时内干燥所得材料。效果：更复杂的技术。 3汤匙

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