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Non-B Hepatitis Virus Genome DNA (DNA) Purification and Comparative Quantitative PCR Method
Non-B Hepatitis Virus Genome DNA (DNA) Purification and Comparative Quantitative PCR Method
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机译:非乙型肝炎病毒基因组DNA(DNA)纯化和比较定量PCR方法
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摘要
The present invention is a method for purifying hepatitis B virus genomic DNA by reacting a microorganism producing hepatitis B virus with a crushed solution containing mercaptoethanol and heating, and the resulting hepatitis B virus genomic DNA and E. coli TrpR gene. By providing a comparative quantitative PCR method for analyzing the degree of inhibition of hepatitis B DNA replication using the present invention, the present invention can be usefully used for the selection of therapeutic agents for hepatitis B.
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