The present invention relates to a method for separating proteins using an AOT / iso-octane reverse micelle system, wherein a protein is extracted into a reverse micelle using a reverse micelle system composed of an anionic surfactant AOT and a non-polar solvent isooctane, AOT / isooctane reverse micelle system which can recover the desired protein from the fermentation broth continuously and on a large scale can be obtained by recovering in liquid form, having a high separation efficiency and purification degree, capable of continuous mass separation, A method for separating proteins used.
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