IDENTIFYING BIOLOGICALLY ACTIVE AGENTS THROUGH CULTURE COLOR CHANGE

摘要

The present invention relates to a method of identifying a biologically active agent, which affects a targeted cellular component, whose existence or function is essential for cell viability, pathogenesis or drug resistance. This assay method includes the use of a mixed culture of first and second cell strains. The cell strains may be either strains of different cells or different strains of the same cell type. The two cell strains are distinguished by color differences. The first cell strain has a first color while the second cell strain displays a second color. The second cell strain is also more sensitive towards a class of biologically active agents than is the first cell strain. The first and second cell strains, and a candidate agent are mixed with a medium, appropriate for growth of the strains, forming a mixed assay culture. The culture is then exposed to conditions appropriate for growth. The initial proportions of the first and second cell strains are such that the culture predominantly favors the development of the second color. The biological activity of the agent is then determined from the color of the culture. Where the mixed culture retains the second color, the agent was not active against the target. However, where the culture predominantly becomes the first color, then the agent showed specific activity towards the target.

机译：本发明涉及鉴定影响靶细胞组分的生物活性剂的方法，所述靶细胞组分的存在或功能对于细胞生存力，发病机理或药物抗性是必不可少的。该测定方法包括使用第一和第二细胞株的混合培养物。细胞株可以是不同细胞株或相同细胞类型的不同株。两种细胞株通过颜色差异来区分。第一细胞株具有第一颜色，而第二细胞株显示第二颜色。与第一细胞株相比，第二细胞株对一类生物活性剂也更敏感。将第一和第二细胞菌株以及候选试剂与适合于菌株生长的培养基混合，形成混合测定培养物。然后将培养物暴露于适合生长的条件。第一和第二细胞株的初始比例应使培养物主要有利于第二种颜色的发展。然后从培养物的颜色确定试剂的生物活性。在混合培养物保留第二种颜色的情况下，该试剂对目标没有活性。但是，在培养物主要成为第一种颜色的地方，则试剂对目标显示出特定的活性。

著录项

公开/公告号WO9506132A2

专利类型
公开/公告日1995-03-02

原文格式PDF
申请/专利权人 MYCO PHARMACEUTICALS INCORPORATED;UNIVERSITY OF GEORGIA RESEARCH FOUNDATION INCORPORATED;
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申请/专利号WO1994US09304
发明设计人 OPPENHEIM AMOS;TIMBERLAKE WILLIAM E.;MORRIS N. RONALD;KOLTIN YIGAL;OCONNOR SEAN CHRISTOPHER;ROTHSTEIN DAVID MICHAEL;GOLDWAY MARTIN;BULAWA CHRISTINE E.;ANDRIANOPOULOS ALEX;
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申请日1994-08-19
分类号C12Q1/02;C12Q1/18;C12Q1/68;C12N1/16;C12N1/19;
国家 WO
入库时间 2022-08-22 04:14:51

IDENTIFYING BIOLOGICALLY ACTIVE AGENTS THROUGH CULTURE COLOR CHANGE

摘要

著录项

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