首页> 外国专利> YEAST STRAIN FOR THE CO-EXPRESSION OF A PLANT CYTOCHROME P450 MONO-OXYGENASE ACTIVITY AND AN ENDOGENOUS OR HETEROLOGOUS NADPH-CYTOCHROME P450-REDUCTASE, AND USE THEREOF IN BIOCONVERSION

YEAST STRAIN FOR THE CO-EXPRESSION OF A PLANT CYTOCHROME P450 MONO-OXYGENASE ACTIVITY AND AN ENDOGENOUS OR HETEROLOGOUS NADPH-CYTOCHROME P450-REDUCTASE, AND USE THEREOF IN BIOCONVERSION

机译：用于共同表达植物细胞色素P450单氧合酶活性和内源性或异源NADPH细胞色素P450还原酶的酵母菌株，并将其用于生物转化

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摘要

A yeast strain for the co-expression of a plant cytochrome P450 mono-oxygenase activity and an endogenous or heterologous NADPH-cytochrome P450-reductase, wherein one of the NADPH-cytochrome P450-reductase or cytochrome P450 genes is integrated into the chromosome of said strain, and the strain is transformed by a vector having an expression cassette for the other gene. The use of said yeast strain, as well as a cDNA sequence coding for cytochrome P450 CA4H of the Jerusalem artichoke Helianthus tuberosus or Arabidopsis thaliana, for bioconversion purposes, is also disclosed.

机译：用于共表达植物细胞色素P450单加氧酶活性和内源或异源NADPH-细胞色素P450-还原酶的酵母菌株，其中NADPH-细胞色素P450-还原酶或细胞色素P450基因之一整合到所述染色体中菌株，然后用具有另一个基因表达盒的载体转化菌株。还公开了所述酵母菌株以及编码菊芋菊芋或拟南芥的细胞色素P450 CA4H的cDNA序列用于生物转化的用途。

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公开/公告号WO9401564A1

专利类型
公开/公告日1994-01-20

原文格式PDF
申请/专利权人 ORSAN;KAZMAIER MICHAEL;POMPON DENIS;MIGNOTTE VIEUX CLAUDIA;TEUTSCH HERMANN;WERK-REICHART DANIELLE;RENAUD MICHEL;
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申请/专利号WO1993FR00676
发明设计人 RENAUD MICHEL;TEUTSCH HERMANN;POMPON DENIS;MIGNOTTE VIEUX CLAUDIA;WERK-REICHART DANIELLE;KAZMAIER MICHAEL;
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申请日1993-07-02
分类号C12N15/53;
国家 WO
入库时间 2022-08-22 04:40:56

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