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Analysis for contaminating biotoxins of mussels, clams and cultivated oysters utilising primary cultures of rat cerebellum neurones
Analysis for contaminating biotoxins of mussels, clams and cultivated oysters utilising primary cultures of rat cerebellum neurones
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机译：利用大鼠小脑神经元原代培养物分析贻贝，蛤和养殖牡蛎的生物毒素污染
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摘要
Analysis for contaminating biotoxins of mussels, clams and cultivated oysters utilising primary cultures of rat cerebellum neurones, these being treated in duplicate with samples of mollusc extract. The toxicity of each extract is evaluated based on the neurotoxic effects caused by four dilutions of same: 0, 10, 50 and 100, corresponding to concentrations of the biotoxins, okadaic acid and domoic acid, greater than, equal to, and less than the legal limit in mollusc tissue. The analysis is done in presence of two times 10exp-6 molar MK801 to selectively visualise the neurotoxicity of okadaic and domoic acid. The toxicity of each extract is evaluated by observing the cultures under the phase contrast microscope after 30 minutes of treatment for domoic acid and 24 hours for okadaic acid. The toxicity can be verified by the combined staining of the neurones with a vital fluorescent dye and with ethidium bromide.
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