New Protein capable of Inducing an immunoprotective Response against Toxoplasma gondii Infection and to Clone and Express a gene encoding the Protein.

• 1. 新的分布在内质网的蛋白质和含有该蛋白质的糖蛋白异常症治疗药、以及用于编码该蛋白质的DNA以及含有该DNA的治疗糖蛋白异常症的基因治疗药 [P] . 中国专利： CN1413251A . 2003-04-23
• 2. 新蛋白质,编码该蛋白质的基因和它们的使用方法 [P] . 中国专利： CN101851281B . 2012.07.04
• 3. PROCESS FOR THE PREPARATION OF A PROTEIN CAPABLE OF INDUCING AN IMMUNE RESPONSE PROTECTING A PROTEIN CAPABLE OF INDUCING AN IMMUNE RESPONSE PROTECTIVE AGAINST INFECTIONS CAUSED BY TOXOPLASMA GONDII [P] . PT99842A . 1992-12-31

  机译： 制备能够诱导免疫应答的蛋白的过程制备能够诱导免疫弓形虫引起的感染的蛋白的过程

• 4. CLONING OF GENE ENCODING THE GP28.5 PROTEIN OF TOXOPLASMA GONDII; PEPTIDE FRAGMENTS OF SAID PROTEIN AND THEIR APPLICATIONS [P] . CA2137280C . 2005-12-27

  机译： 弓形虫GP28.5蛋白基因的克隆赛德蛋白的肽片段及其应用

• 5. CLONING OF GENE ENCODING THE GP28.5 PROTEIN OF TOXOPLASMA GONDII; PEPTIDE FRAGMENTS OF SAID PROTEIN AND THEIR APPLICATIONS [P] . 欧洲知识产权局专利： EP0648270B1 . 2005-12-28

  机译： 弓形虫GP28.5蛋白基因的克隆赛德蛋白的肽片段及其应用

• 1. Amplification，cloning，sequencing　and　expression　of　the　gene　encoding　the　major　surface　antigen　of　Toxoplasma　gondii　isolated [J] . 陈晓光, 刘国章, 徐帆, 中国人民解放军军医大学学报：英文版 . 1994,第002期
• 2. Molecular cloning and expression analysis of the Rf-m candidate genes encoding pentatricopeptide repeat proteins in soybean [J] . Dagang Wang, Shengnan Chen, Jiekun Li, 中国油料作物学报（英文版） . 2021,第003期
• 3. Molecular cloning and expression analysis of the Rf-m candidate genes encoding pentatricopeptide repeat proteins in soybean [J] . Dagang Wang, Shengnan Chen, Jiekun Li, 中国油料作物学报（英文） . 2021,第003期
• 4. Expression of p30, the major surface antigen of Toxoplasma gondii, in baculovirus-insect cell system and the evaluation of immune response induced by p30 [J] . 陈晓光, 陈兆明, 马鑫, 中国人民解放军军医大学学报：英文版 . 2000,第003期
• 5. Cloning and molecular analysis of Toxoplasma gondii Surface Antigen 2(SAG2)gene cloned from Toxoplasma gondii DNA isolated from Javanese acute toxoplasmosis [C] . W Monica, A A Prasetyo, S Haryati Annual Applied Science and Engineering Conference . 2019
• 6. An investigation of Erysipelothrix rhusiopathiae isolates affecting marine mammals and the cloning, expression and evaluation of a putative recombinant 66 kD immunoprotective surface protein. [D] . Osgood, Robert Charles. 2004
• 7. Virus-Like Particles Expressing Toxoplasma gondii Rhoptry Protein 18 Induces Better Protection Than Rhoptry Protein 4 against T. gondii Infection [O] . Hae-Ji Kang, Su-Hwa Lee, Ki-Back Chu, 2018
• 8. Isolation of cDNA clones for genes that are expressed during leaf senescence in Brassica napus. Identification of a gene encoding a senescence-specific metallothionein-like protein. [O] . Buchanan-Wollaston, V 1994

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. 一个新的原癌基因编码的四次穿膜蛋白质LAPTM4β——肿瘤治疗中的新靶标 [C] . 周柔丽 ,邵根泽 ,刘歆荣 . 第八届海内外生命科学论坛 . 2004
• 3. 隐藏的人类蛋白质组：“非编码”RNAs编码的新蛋白质 [A] . 卢少华 . 2016