首页> 外国专利> Prodn. of potato plants with suppressed tuber sprouting - by genetic modification to reduce sucrose concn.

Prodn. of potato plants with suppressed tuber sprouting - by genetic modification to reduce sucrose concn.

机译：产品抑制块茎发芽的马铃薯植物的育种-通过基因修饰减少蔗糖浓度。

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Genetically modified potato plants in which tuber sprouting is suppressed are produced by a process in which the concn. of sprout-inducing sucrose is reduced by inhibition of starch-degrading enzymes and/or by degradation of sucrose already formed, by means of a recombinant double-stranded DNA mol. (I) which is localised in a plasmid and integrated into the genome of potato plants. (I) comprises: (a) a promoter that is functional in plant cells; (b) at least one coding sequence in sense and/or antisense orientation which can be coupled to a signal sequence and is fused to the promoter in such a way that the coding or noncoding strand can be read; and (c) a transcription termination and polyadenylation signal sequence that is functional in plants. Also claimed are: (1) the use of plasmid p35S-CW-INV (DSM 5788), which contains an invertase gene, to produce transgenic potato plants in which the mobilisation of starch in the tuber storage tissue is suppressed to improve the storage properties of the tubers and inhibit sprouting; (2) the use of plasmid p35S-CW-INV to produce potato plants in which enrichment of reducing sugars in the tubers at storage temps. above 8 deg.C is inhibited; (3) potato tubers whose storage properties are improved by suppression of sprouting; and (4) the use of tubers (3) to produce potato plants whose tubers have improved storage properties.

机译：通过浓缩的过程产生了抑制块茎发芽的转基因马铃薯植物。借助于重组双链DNA mol，通过抑制淀粉降解酶和/或通过降解已经形成的蔗糖，可以减少诱导芽苗的蔗糖的量。（I）位于质粒中并整合到马铃薯植物的基因组中。（I）包括：（a）在植物细胞中有功能的启动子; （b）至少一个在有义和/或反义方向上的编码序列，其可以与信号序列偶联并且以可以读取编码或非编码链的方式与启动子融合; （c）在植物中起作用的转录终止和聚腺苷酸化信号序列。还要求保护的是：（1）含有转化酶基因的质粒p35S-CW-INV（DSM 5788）在生产转基因马铃薯植株中的应用，其中马铃薯块茎贮藏组织中的动员被抑制以改善贮藏性能块茎，抑制发芽; （2）使用质粒p35S-CW-INV生产马铃薯植株，其中马铃薯在储存温度下富含还原糖。高于8摄氏度被抑制; （3）通过抑制发芽来提高贮藏性能的马铃薯块茎; （4）使用块茎（3）来生产块茎具有改善的贮藏特性的马铃薯植株。

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公开/公告号DE4213444A1

专利类型
公开/公告日1993-10-28

原文格式PDF
申请/专利权人 INSTITUT FUER GENBIOLOGISCHE FORSCHUNG BERLIN GMBH 1000 BERLIN DE;
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申请/专利号DE19924213444
发明设计人 WILLMITZER LOTHAR PROF. DR. 1000 BERLIN DE;SONNEWALD UWE 1000 BERLIN DE;SCHAEWEN ANTJE VON 4500 OSNABRUECK DE;
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申请日1992-04-18
分类号A01H5/00;C12N15/29;C12N15/82;C12N15/56;C12N5/10;A01H1/06;
国家 DE
入库时间 2022-08-22 05:01:18

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