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DNA FRAGMENT ENCODING NON-A NON-B TYPE HEPATITIS SPECIFIC ANTIGEN, ITS MANIFESTATION AND METHOD FOR DETECTING NON-A NON-B TYPE HEPATITIS VIRUS

机译：编码非A型非B型肝炎特异性抗原的DNA片段及其检测方法及检测方法

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摘要

PURPOSE:To obtain a new DNA fragment capable of diagnosing non-A non-B type hepatitis in high accuracy. CONSTITUTION:A DNA fragment which is a DNA fragment derived and obtained by genetic engineering from non-A non-B type hepatitis virus RNA directly collected from plasma of a patient of non-A non-B type hepatitis and contains a base sequence encoding a non-A non-B type hepatitis specific antigen polypeptide derived from the virus nonstructural protein. For example, the base sequence is shown by an amino acid sequence encoded according to a reading frame of the formula. A total RNA containing a non-A non-B type hepatitis virus RNA is taken out from a fresh plasma pool of a Japanese patient of a non-A non-B type hepatitis in a chronic stage and a cDNA library prepared by integrating a lambda phage to a cDNA synthesized by random primer method is subjected to immuno screening with serum of a patient of a non-A non-B type hepatitis to give a new DNA fragment.

机译：目的：获得能够高精度诊断非甲型非乙型肝炎的新DNA片段。组成：一个DNA片段，是通过基因工程从非A型非B型肝炎病毒RNA中直接获得的DNA片段，该RNA直接从非A型非B型肝炎患者的血浆中收集，并包含编码源自病毒非结构蛋白的非A非B型肝炎特异性抗原多肽。例如，碱基序列由根据式的阅读框编码的氨基酸序列表示。从日本慢性非A非B型肝炎患者的新鲜血浆库中提取含有非A非B型肝炎病毒RNA的总RNA和通过整合lambda制备的cDNA文库用非A非B型肝炎患者的血清对通过随机引物法合成的cDNA的噬菌体进行免疫筛选，以得到新的DNA片段。

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公开/公告号JPH0584085A

专利类型
公开/公告日1993-04-06

原文格式PDF
申请/专利权人 TONEN CORP;
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申请/专利号JP19910189268
发明设计人 MAKI NOBORU;TOYOSHIMA AYUMI;OBARA MICHINORI;YAMAGUCHI KENJIRO;
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申请日1991-07-03
分类号C12N15/51;C07H21/04;C07K13/00;C12N1/21;C12N15/10;C12N15/11;C12N15/71;C12P21/02;C12P21/08;C12Q1/68;G01N33/53;G01N33/569;G01N33/576;
国家 JP
入库时间 2022-08-22 05:13:35

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