首页> 外国专利> METHOD OF OBTAINING THE EXPRESSION OF HETEROLOG GEN AND SECRETION OF PROTEIN, METHOD OF ACTIVATING THE REGULATOR REGION OF ACID PHOSPHATASE, METHOD OF OBTAINING A PLASMIDE AND AN EXPRESION VECTOR FOR THE TISSULAR PLASMINOGEN ACTIVATOR, METHOD OF DIRECTING THE INTEGRATION INTO THE LOCUS PHO1 PICHIA PASTORIS AND METHOD OF IDENTIFYING THE TRANSFORMANTS OF PICHIA PASTORIS

METHOD OF OBTAINING THE EXPRESSION OF HETEROLOG GEN AND SECRETION OF PROTEIN, METHOD OF ACTIVATING THE REGULATOR REGION OF ACID PHOSPHATASE, METHOD OF OBTAINING A PLASMIDE AND AN EXPRESION VECTOR FOR THE TISSULAR PLASMINOGEN ACTIVATOR, METHOD OF DIRECTING THE INTEGRATION INTO THE LOCUS PHO1 PICHIA PASTORIS AND METHOD OF IDENTIFYING THE TRANSFORMANTS OF PICHIA PASTORIS

机译：获得异源基因的表达和蛋白分泌的方法，激活酸性磷酸酶的调节剂区域的方法，为组织性纤溶酶原激活剂获得一种磷脂和一种表达载体的方法，将注入的内毒素和输入到体内的一种方法鉴定巴斯德毕赤酵母转化体的方法

页面导航

摘要
著录项
相似文献

摘要

The present invention discloses the Pichia pastoris acid phosphatase gene, which includes the 5' regulatory region, signal sequence, structural gene, and 3' transcription termination sequence. Also disclosed are methods of using these fragments, which include but are not limited to the secretion of proteins from cells and the regulation of the transcription of DNA. DNA vectors containing the acid phosphatase gene or fragments thereof and hosts transformed with these vectors are also disclosed. Additionally, integrative vectors which direct integration at the Pichia pastoris PHO1 locus and a method of identifying these disruptants is disclosed.

机译：本发明公开了巴斯德毕赤酵母酸性磷酸酶基因，其包括5'调节区，信号序列，结构基因和3'转录终止序列。还公开了使用这些片段的方法，包括但不限于从细胞分泌蛋白质和调节DNA的转录。还公开了含有酸性磷酸酶基因或其片段的DNA载体和用这些载体转化的宿主。另外，公开了指导在巴斯德毕赤酵母PHO1基因座处整合的整合载体和鉴定这些破坏物的方法。

著录项

公开/公告号PL292765A1

专利类型
公开/公告日1992-08-24

原文格式PDF
申请/专利权人 PHILLIPS PETROLEUM CO;
展开▼

申请/专利号PL19910292765
发明设计人 BUCKHOLZ RICHARD G;
展开▼

申请日1991-12-13
分类号C12N15/63;
国家 PL
入库时间 2022-08-22 05:34:20

相似文献

专利
外文文献
中文文献