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METHOD OF FORMING CELLULAR MASS CONTROLLED IN NUMBER OF CELL AND CELL CULTIVATION MEDIUM USED THEREFOR
METHOD OF FORMING CELLULAR MASS CONTROLLED IN NUMBER OF CELL AND CELL CULTIVATION MEDIUM USED THEREFOR
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机译：形成受细胞数量控制的细胞质量的方法及其所用的细胞培养培养基
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摘要
PURPOSE: To efficiently obtain a cell culture base, by adjusting the surface area of a temperature-sensitive polymer compound and culturing cells. ;CONSTITUTION: A cell nonadherent substance 1 is formed on the surface of a temperature-sensitive polymer compound 2 (A) selected from a poly-N substituted acrylamide derivative, etc. to give a culture base (B). The face for cell proliferation of the base B is coated with a mixture of the component A and an adherent/proliferation factor (e.g. gelatin) of cells 3. Then a linear function of the equation, y=ax (x) is the surface area of the component A; (y) is the number of cells of the component C; and (a) is a cell specific constant) is obtained from a relation between the number of cells of a cell massive plate (C) formed by culturing cells 3 to be subjected to a monolayer culture and the surface area of the component A. Then cells are cultured on the base B composed of the component A having X for a fixed (y) by the equation at a temperature of ≥LCST (hydration/dehydration transition temperature) of the component A, the temperature is dropped lower than LCST, the cells 3 proliferated on the surface of the component A are eliminated from the base B to obtain a cell massive formed product.;COPYRIGHT: (C)1992,JPO
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