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16SRRNA GENE OF BORDETELLA BRONCHISEPTICA, DNA FRAGMENT FOR PROBE AND METHOD FOR DETECTING BORDETELLA BRONCHISEPTICA USING THE SAME
16SRRNA GENE OF BORDETELLA BRONCHISEPTICA, DNA FRAGMENT FOR PROBE AND METHOD FOR DETECTING BORDETELLA BRONCHISEPTICA USING THE SAME
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机译：桔小球藻的16S RRNA基因，探针的DNA片段和使用相同方法检测桔小球藻的方法
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摘要
NEW MATERIAL:The subject gene having a base sequence expressed by the formula. USE:Detection of Bordetella bronchiseptica, etc. PREPARATION:A DNA of Bordetella bronchiseptica is cleaved with BamHI and inserted into lambda phage vector EMBL4 to prepare a DNA library, which is then cloned by a polymerase chain reaction (PCR) method to provide a 16 SrRNA genetic fragment. The resultant genetic fragment is subsequently used as a probe to carry out screening. A alone containing 16SrRNA gene of a complete length is selected. A BamHI-SalI DNA fragment of about 1.7kb containing the 16SrRNA gene of the complete length is obtained from the prepared clone.
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