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manufacture of against arsenic resistant plasmid for thiobacillus ferrooxidans and further arsenresistenz (plasmids

机译:亚铁氧合硫杆菌抗砷抗性质粒的制备及进一步的抗砷性质粒

摘要

A process for constructing the plasmids pDR416 and pDR420 which contain arsenic resistance genes and which are able to replicate in both T. ferroxidans and E. coli comprises preparing a recombinant of pBR325 or the like and a T. ferrooxidans plasmid, forming a deletion plasmid and inserting arsenic resistance genes e.g. from R46 by EcoR1 excision and ligation.
机译:构建含有砷抗性基因并且能够在四氧化三铁和大肠杆菌中复制的质粒pDR416和pDR420的方法包括制备pBR325等的重组体和四氧化三铁质粒,形成缺失质粒和插入砷抗性基因,例如从R46通过EcoR1切除和结扎。

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