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CLONING, EXPRESSION AND SEQUENCING OF AN ESTER HYDROLASE GENE IN ESCHERICHIA COLI
CLONING, EXPRESSION AND SEQUENCING OF AN ESTER HYDROLASE GENE IN ESCHERICHIA COLI
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机译:大肠杆菌中的酯水解酶基因的克隆,表达与序列分析
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摘要
The present invention is directed to the production of an ester hydrolase, in particular, utilizing recombinant molecules containing an ester hydrolase gene from Pseudomonas fluorescens and the generation of a cloned hydrolase and mutants thereof in Escherichia coli. The cloned hydrolases so produced are especially useful in a process in which R-,5-naproxen esters are enzymatically hydrolyzed in an enentiospecific manner.
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