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Purification and characterization of an acetyl-CoA hydrolase

机译:乙酰辅酶A水解酶的纯化和表征

摘要

This invention is directed to the isolation, identification, purification and characterization of acetyl-CoA hydrolase, the enzyme having a molecular weight of about 64,000 daltons and an enzyme activity of greater than 20 wherein one unit of acetyl CoA hydrolase activity is defined as the amount of enzyme able to inhibit 1 unit of acetyltransferase activity wherein 1 unit of acetyltransferase activity is defined as the amount of enzyme able to transfer 1 pmol of [³H] acetyl group from [³H] acetyl coenzyme A to ACTH (1-24) by acetyltransferase standard enzyme assay under standard conditions. This invention is further directed to a method for purifying the acetyl-CoA hydrolase.
机译:本发明涉及乙酰-CoA水解酶的分离,鉴定,纯化和表征,该酶具有约64,000道尔顿的分子量和大于20的酶活性,其中一个单位的乙酰CoA水解酶活性定义为能够抑制1个单位的乙酰转移酶活性的酶的数量,其中1个单位的乙酰转移酶活性定义为能够通过乙酰转移酶将1 pmol的[³H]乙酰基从[³H]乙酰辅酶A转移到ACTH(1-24)的酶的量在标准条件下进行标准酶分析。本发明进一步涉及纯化乙酰辅酶A水解酶的方法。

著录项

  • 公开/公告号EP0348958A2

    专利类型

  • 公开/公告日1990-01-03

    原文格式PDF

  • 申请/专利权人 THE GENERAL HOSPITAL CORPORATION;

    申请/专利号EP19890111804

  • 发明设计人 SMITH JOHN A.;LEE FANG-JEN S.;

    申请日1989-06-29

  • 分类号C12N9/16;C07K7/00;C12N15/55;C12N5/00;A01H1/00;

  • 国家 EP

  • 入库时间 2022-08-22 06:14:00

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