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Purification and characterization of an acetyl-CoA hydrolase
Purification and characterization of an acetyl-CoA hydrolase
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机译:乙酰辅酶A水解酶的纯化和表征
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摘要
This invention is directed to the isolation, identification, purification and characterization of acetyl-CoA hydrolase, the enzyme having a molecular weight of about 64,000 daltons and an enzyme activity of greater than 20 wherein one unit of acetyl CoA hydrolase activity is defined as the amount of enzyme able to inhibit 1 unit of acetyltransferase activity wherein 1 unit of acetyltransferase activity is defined as the amount of enzyme able to transfer 1 pmol of [³H] acetyl group from [³H] acetyl coenzyme A to ACTH (1-24) by acetyltransferase standard enzyme assay under standard conditions. This invention is further directed to a method for purifying the acetyl-CoA hydrolase.
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