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USE OF SITE-SPECIFIC MODIFIED LIPOLYTIC ENZYMES FOR THE SELECTIVE HYDROLYSIS OF ORGANIZED LIPID STRUCTURES

机译:特定位点修饰的脂解酶在有机脂质结构选择性加氢中的应用

摘要

The invention relates to a method of selectively hydrolysing organized phospholipids by means of lipolytic enzymes having regulated penetrating and hydrolising capacity due to site-specific modification with a hydrophobic group. More particularly, useful site-specific acylated pancreatic phospholipases A2 are capable of hydrolysing phospholipids of organized lipid-water interfaces, for example micelles, vesicles, liposomes and other bilayer structures, hexagonal HII-phases or biological membranes. The invention also relates to a method for the preparation of these modified enzymes and to the enzymes in substantially pure form.
机译:本发明涉及一种通过脂解酶选择性水解有组织的磷脂的方法,该脂解酶由于通过疏水基团的位点特异性修饰而具有调节的渗透和水解能力。更特别地,有用的位点特异性酰化胰磷脂酶A2能够水解有组织的脂质-水界面的磷脂,所述脂质-水界面例如为胶束,囊泡,脂质体和其他双层结构,六方HII相或生物膜。本发明还涉及制备这些修饰的酶的方法和基本上纯的形式的酶。

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