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Method for controlling the expression of a foreign gene in Escherichia coli and methods for producing a foreign gene product
Method for controlling the expression of a foreign gene in Escherichia coli and methods for producing a foreign gene product
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机译:控制大肠杆菌中外源基因表达的方法和生产外源基因产物的方法
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摘要
Escherichia coli carrying a hybrid plasmid having been constructed by inserting a desired foreign gene into an expression vector so as to permitting expression of said desired foreign gene therein was cultured at a temperature 40 DEG C or over so that the expression of said desired foreign gene was suppressed. This E. coli (i.e., transformant) was cultured at 40 DEG C or over in a first process to suppress the expression of the foreign gene and to support sufficient cell growth and thereafter below 40 DEG C in a second process to release the suppression of the expression so as to permit effective production of the foreign gene product, which resulted in high concentration of the foreign gene product in the final culture.
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