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DETERMINATION OF NUMBER OF METHANOGEN CELLS OR METHANE PRODUCTION ACTIVITY OF METHANOGEN
DETERMINATION OF NUMBER OF METHANOGEN CELLS OR METHANE PRODUCTION ACTIVITY OF METHANOGEN
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机译：甲醇细胞的数量或甲烷的甲烷生产活性的测定
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摘要
PURPOSE:To determine the number of methanogen cells or the methane-production activity of methanogen, in high sensitivity, by heating a specimen containing methanogen, irradiating the specimen with excitation light of a specific wavelength range, and measuring the intensity of fluorescent light emitted from the specimen and having a specific wavelength range. CONSTITUTION:A specimen containing methanogen is heated at 60-120 deg.C, irradiated with excitation light of a specific wavelength range. e.g. 380-440nm, and the intensity of fluorescent light emitted from the specimen and having a specific wavelength range is measured. In the above process, the fluorescent light having the above wavelength range is emitted from the F420 substance inherent to the methanogen and existing in the electron transfer system in the energy metabolic system of the methanogen. The number of the methanogen cells or the methane-production activity of methanogen can be determined by this process. The measurement can be carried out even in a mixture of microorganisms containing foreign materials such as the digested sludge in a methane fermentation tank, etc.
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