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DNA ENCODING STREPTAVIDIN, STREPTAVIDIN PRODUCED THEREFROM, FUSED POLYPEPTIDES WHICH INCLUDE AMINO ACID SEQUENCES PRESENT IN STREPTAVIDIN AND USES THEREOF
DNA ENCODING STREPTAVIDIN, STREPTAVIDIN PRODUCED THEREFROM, FUSED POLYPEPTIDES WHICH INCLUDE AMINO ACID SEQUENCES PRESENT IN STREPTAVIDIN AND USES THEREOF
DNA which encodes the polypeptide streptavidin has been isolated as a fragment 2kb in length derived from a restriction endonuclease digestion of the chromosomal DNA of Streptomyces avidinii. The nucleic acid sequence of the gene and the amino acid sequence of the polypeptide have been determined. A fused gene has been prepared which comprises the streptavidin gene fused to a gene encoding the human LDL receptor. Expression of the gene fusion results in a fused streptavidin-human LDL receptor polypeptide. Methods are provided for using the fused gene to produce labeled, chemically modified proteins in vivo and to isolate a protein knowing only the nucleotide sequence of the gene encoding the protein.
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