首页>
外国专利>
TEST KIT FOR FREE SPECIES QUANTITATIVE ANALYSIS AND METHOD THEREFOR
TEST KIT FOR FREE SPECIES QUANTITATIVE ANALYSIS AND METHOD THEREFOR
展开▼
机译：用于免费物种的测试套件定量分析及其方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
Disclosed is a process for determining the concentration of unbound hormone, e.g., thyroxine, cortisol, or testosterone, in a liquid sample containing hormone and protein capable of binding the hormone. The sample is incubated with antibody specific to the hormone to be detected and a distinguishable analogue of the hormone, both of which are separated from the sample by semipermeable membranes capable of excluding the passage of natural protein and antibody but which allow passage of the hormone and its analogue. Preferably, the antibody and analogue are contained in semipermeable microcapsules. Free hormone in the sample permeates the membranes and competes for sites of attachment to the antibody with the analogue. The antibody and free hormones are then separated. The amount of analogue present either in association with the antibody or in the remainder of the reaction system is indicative of the level of free hormone originally present in the sample.
展开▼
