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METHOD FOR OBTAINING HIGHLY PRODUCTIVE STRAIN FOR PROTEIN AND PRODUCTION OF PROTEIN USING SAID HIGHLY PRODUCTIVE STRAIN
METHOD FOR OBTAINING HIGHLY PRODUCTIVE STRAIN FOR PROTEIN AND PRODUCTION OF PROTEIN USING SAID HIGHLY PRODUCTIVE STRAIN
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机译:获得蛋白质的高产菌株的方法和使用所述高产菌株的蛋白质生产
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摘要
PURPOSE:To obtain a strain capable of highly producing a specific protein, by transfecting a DNA having a gene sequence coding a protein into a host higher eucaryotic cell having a specific gene sequence in the chromosomic DNA. CONSTITUTION:A DNA having a gene sequence I for coding a specific protein, gene sequence II for coding dihydrofolate reductase derived from wild-type cells and gene sequence III for coding a protein for sorting out a transformant is transfected into a host higher eucaryotic cell having a gene sequence for coding a methotrexate-sensitive dihydrofolate reductase in the chromosomic DNA. The resultant transformant strain is then cultivated in the presence of a competitive inhibitor having dihydrofolate reductase activity to amplify both the gene sequences I and II at the same time.
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