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Adult schistosome worm-derived antigenic substance and method of obtaining same

机译:成人血吸虫蠕虫来源的抗原物质及其获得方法

摘要

An extract of adult Schistosome mansoni worms, obtained by incubation in 0.15 M sodium chloride-sodium phosphate buffer (pH 6.8), contains protein, carbohydrates, and nucleic acid and/or by-products of the latter component and resolves into four major fractions by gel chromatography in G-100 and G-200 Sephadex columns. Immunodiffusion tests with rabbit anti-total extract serum reveal three precipitation lines corresponding to fractions I and II, and one with III or IV. Rabbits immunized with this total extract are found to be totally or partially (at least 77%) resistant to a challenge infection. The saline extract antigenic material is an effective vaccine for the treatment and immunization of schistosomiasis and other schistosome infections.
机译:通过在0.15 M氯化钠-磷酸钠缓冲液(pH 6.8)中孵育获得的成人血吸虫曼氏蠕虫提取物,其中含有蛋白质,碳水化合物和核酸和/或后一成分的副产物,并通过分解为四个主要部分G-100和G-200 Sephadex柱进行凝胶色谱分析。用兔抗总提取物血清进行的免疫扩散测试显示,对应于级分I和II的三个沉淀线,以及具有III或IV的沉淀线。发现用该总提取物免疫的兔子对攻击性感染具有全部或部分抗性(至少77%)。盐水提取物抗原物质是用于治疗和免疫血吸虫病和其他血吸虫病感染的有效疫苗。

著录项

  • 公开/公告号US4396600B1

    专利类型

  • 公开/公告日1986-02-11

    原文格式PDF

  • 申请/专利权人

    申请/专利号US19800217575

  • 发明设计人

    申请日1980-12-18

  • 分类号A61K39/002;

  • 国家 US

  • 入库时间 2022-08-22 07:29:53

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