首页> 外国专利> The human T cell leukemia in the bacterium (direction lymph characteristic) the retro-virus (HTLVI) production of the enbero - pu protein fragment and the use null in serum epidemiology searching of the human lymph type malignant conversion

The human T cell leukemia in the bacterium (direction lymph characteristic) the retro-virus (HTLVI) production of the enbero - pu protein fragment and the use null in serum epidemiology searching of the human lymph type malignant conversion

机译:细菌中的人类T细胞白血病(定向淋巴特征)enbero-pu蛋白片段的逆转录病毒(HTLVI)产生以及在人类流行病学中寻找人类淋巴型恶性转化的无效性

摘要

Two regions of the gene for the HTLV-I envelope were expressed in Escherichia coli by use of the vector pJLA16. One corresponds to the carboxy-terminal region of the major envelope protein p46 and the other corresponds to the transmembrane protein p21E. Reactivity of the expressed protein with human sera was tested by Western blot procedure. Each of the sera tested that had been shown to contain anti-HTLV-I or anti-HTLV-II antibodies by ELISA assay recognized the bacterially synthesized envelope proteins. There was no reaction detected when control sera were tested. This system is useful for large scale seroepidemiological surveys for this and related human retroviruses.
机译:通过使用载体pJLA16在大肠杆菌中表达HTLV-1包膜基因的两个区域。一个对应于主要包膜蛋白p46的羧基末端区域,另一个对应于跨膜蛋白p21E。通过蛋白质印迹法测试表达的蛋白质与人血清的反应性。通过ELISA测定显示出包含抗HTLV-I或抗HTLV-II抗体的每个被测血清都可以识别细菌合成的包膜蛋白。测试对照血清时未检测到反应。该系统可用于这种和相关人类逆转录病毒的大规模血清流行病学调查。

著录项

  • 公开/公告号JPS61502028A

    专利类型

  • 公开/公告日1986-09-18

    原文格式PDF

  • 申请/专利权人 US HEALTH;

    申请/专利号JP19850504883

  • 发明设计人

    申请日1985-10-24

  • 分类号G01N33/53;A61K39/21;C07K14/005;C07K14/15;C07K14/195;C12N15/00;C12N15/09;C12P21/00;C12P21/02;C12Q1/00;C12Q1/68;C12Q1/70;C12R1/19;G01N33/531;G01N33/569;G01N33/574;G01N33/577;

  • 国家 JP

  • 入库时间 2022-08-22 07:44:49

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