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Monoclonal antibodies antipoliovirus for the identification of the type and differenciation intratypic of strains isolated of poliovirus, cellular hybrids for their manufacture and in vitro diagnostic using antibodies
Monoclonal antibodies antipoliovirus for the identification of the type and differenciation intratypic of strains isolated of poliovirus, cellular hybrids for their manufacture and in vitro diagnostic using antibodies
Monoclonal antibody collection for the diagnosis and identification of poliovirus contains three groups of poliovirus antibodies, related respectively to the serological sub-types (1), (2) or (3). The monoclonal antibodies of each group may be divided into (a) a first category of active antibodies, but which do not show intratypic differentiation; (b) a second category of antibodies active against the attenuated strains, but essentially devoid of activity against the corresponding natural strains and (c) a third category of antibodies active against the natural strain of the serological sub-type being considered, but essentially devoid of activity w.r.t. the corresponding attenuated strains. The antibodies of the first and second categories of each of the above three groups respectively are active against LSc 2ab strain (sero-group 1), P 712 CH 2ab strain (group 2), and Leon 12alb strain (group 3). The antibodies of first and third categories in each of the above three groups respectively are active against Mahoney strain (Group 1), MEF 1 strain (Group 2) and Saukett strain (Group 3). Diagnosis and identification of newly isolated unknown strains of poliovirus as being natural or attenuated types. In the first stage of seroneutralisation tests with sensitive cell cultures in the presence of the three-monoclonal antibodies of the first category of the three groups, the virus is presumed to belong to the first, second or third type according to whether it is inhibited by the antibodies of the first, second or third group. In the second stage, the seroneutralisation tests are repeated under similar conditions but using antibodies of the second category or the third category of the group corresponding to the serotype shown in the first stage tests; the virus being determined as attenuated or natural depending on whether it inhibits the antibodies of the second or third category.
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