首页> 外国专利> Cloning and expression vehicle for yeast - contg. heterologous gene and plasmid k1 of Kluyveromyces lactis

Cloning and expression vehicle for yeast - contg. heterologous gene and plasmid k1 of Kluyveromyces lactis

机译:酵母的克隆和表达载体-续乳酸克鲁维酵母的异源基因和质粒k1

摘要

Vector for cloning and expressing a heterologous gene in a yeast comprises (1) all or part of the DNA of the k1 plasmid of Kluyveromyces lactis, and (2) a DNA fragment contg. the heterologous gene plus appropriate expression sequences. Partic. component (2) is a segment of the URA3 gene of Saccharomyces cerevisiae, partic. a 1.1 kb fragment with Hind III sites at both ends. This fragment is introduced into an EcoR1, BamHI ClaI or Hind III site of the k1 plasmid. Also new are yeasts (esp. K. lactis) transformed with this vector. The transformants express the protein coded for by the heterologous gene. These vectors contain restriction sites of many different types; can be amplified in E. coli and is easily extracted.
机译:用于在酵母中克隆和表达异源基因的载体包括(1)乳酸克鲁维酵母k1质粒的全部或部分DNA,和(2)连续的DNA片段。异源基因加上适当的表达序列。参加组分(2)是酿酒酵母(部分)的URA3基因的片段。一个在两端都有Hind III位点的1.1 kb片段。将该片段引入k1质粒的EcoR1,BamHI ClaI或Hind III位点。用该载体转化的酵母(尤其是乳酸克鲁维酵母)也是新的。转化体表达异源基因编码的蛋白质。这些载体包含许多不同类型的限制性酶切位点。可在大肠杆菌中扩增并易于提取。

著录项

  • 公开/公告号JPS59501045A

    专利类型

  • 公开/公告日1984-06-21

    原文格式PDF

  • 申请/专利权人

    申请/专利号JP19830501820

  • 发明设计人

    申请日1983-06-01

  • 分类号C12N15/00;C12N1/16;C12N1/19;C12N15/09;C12N15/81;

  • 国家 JP

  • 入库时间 2022-08-22 09:35:29

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