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Cloning and expression vehicle for yeast - contg. heterologous gene and plasmid k1 of Kluyveromyces lactis
Cloning and expression vehicle for yeast - contg. heterologous gene and plasmid k1 of Kluyveromyces lactis
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机译:酵母的克隆和表达载体-续乳酸克鲁维酵母的异源基因和质粒k1
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摘要
Vector for cloning and expressing a heterologous gene in a yeast comprises (1) all or part of the DNA of the k1 plasmid of Kluyveromyces lactis, and (2) a DNA fragment contg. the heterologous gene plus appropriate expression sequences. Partic. component (2) is a segment of the URA3 gene of Saccharomyces cerevisiae, partic. a 1.1 kb fragment with Hind III sites at both ends. This fragment is introduced into an EcoR1, BamHI ClaI or Hind III site of the k1 plasmid. Also new are yeasts (esp. K. lactis) transformed with this vector. The transformants express the protein coded for by the heterologous gene. These vectors contain restriction sites of many different types; can be amplified in E. coli and is easily extracted.
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