Disclosed is a method of detecting in a human the subject's extent of immunity to malignant tumor development, by (a) intradermally injecting into the subject an aqueous isotonic diagnostic solution of (i) a polysaccharide-like antigenic product obtained by rupturing the cells of malignant tumor tissue as by disintegration in hypertonic aqueous saline solution, removing and discarding the nuclear and cell wall fractions, separating from the residual liquid the mitochondrial fraction by a treatment including centrifugation, removing from that fraction lipid and protein materials, and dialyzing the remainder against isotonic phosphate saline solution, or (ii) the produce derived from the chromatographically treating the former product and combining selected fractions of an eluate, and (b) measuring separately the two longest diameters perpendicular to one another of the erythema produced at the injection site, and (c) calculating their average and from its value determining the level of immunity from a respective value level of immunity scale. How the respective polysaccharide-like antigenic products are obtained also is disclosed.
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