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Live vaccine against theileriosis in cattle - contg. lymphoid cells infested with attenuated strain of Theileria annulata

机译:牛抗回肠病的活疫苗-续环状泰勒菌减毒株感染的淋巴细胞

摘要

Live vaccine against theileriosis in cattle consists of cattle lymphoid cells infested with Schizota of the attenuated strain Theileria annulata KTa No. 7. Pref. the vaccine is administered as a dose contg. 0.1-3 million living, infested clles. The vaccine is prepd. by growing T. annulata CY-1 (isolated from the mite Hyalomma detritum) on cattle lymphoid cells in a nutrient medium for 20-100 days, esp. in 6-12 passages over 60-80 days at 37.5-38.5 degrees C. The schizota are then collected and frozen in presence of a suitable cryoprotectant, esp. glycerine, at is not -60 degrees C. The vaccine has high immunogenicity and is 10 times cheaper than known vaccines. It is only weakly reactogenic and induces no side effects in vaccinated cattle.
机译:牛抗狂犬病的活疫苗由被减毒菌株无环泰勒氏菌KTa 7号的裂殖体侵染的牛淋巴样细胞组成。疫苗以连续剂量给药。 0.1-3百万个活着,受感染的克莱尔。准备疫苗。通过在营养培养基中的牛淋巴细胞上生长无花线虫CY-1(从螨虫透明质体中分离出)20-100天,特别是。在60℃至80天内在37.5℃至38.5℃下经过6-12次传代。然后收集裂殖体并在合适的冷冻保护剂特别是存在下冷冻。甘油温度不高于-60摄氏度。该疫苗具有很高的免疫原性,并且比已知疫苗便宜10倍。它在疫苗接种的牛中仅具有弱的反应原性,不会引起任何副作用。

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