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Measuring sensitivity of microparticles to outside influences - esp. bacteria to antibiotics, by comparing light scattering patterns of treated and control samples
Measuring sensitivity of microparticles to outside influences - esp. bacteria to antibiotics, by comparing light scattering patterns of treated and control samples
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机译：测量微粒对外部影响的敏感性-尤其是通过比较处理样品和对照样品的光散射模式，将细菌与抗生素结合
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摘要
Testing the sensitivity of microparticles (M) to environmental influences comprises (a) preparing an M-control sample; (b) placing this in the path of a thin, monochromatic beam of electromagnetic radiation; (c) measuring the intensity of the scattered radiation at a number of different angles of sample to beam direction so as to generate a differential control scattering pattern (P1); (d) repeating this procedure for an M sample which has been subjected to some alteration in its environment to generate a differential test scattering pattern (P2), (e) displacing P2 along a chosen axis relative to P1 to minimise the algebraic sum of the areas between the two; and (e) comparing the two patterns, any difference being a measure of the sensitivity of S to the environmental change. An appts. for carrying out this is also claimed. Esp. useful for determining the effects of antibiotics on bacteria but M may also be mammalian cells, viruses, antibodies etc. The method is more sensitive than turbidimetry and nephelometry esp. for bacteria with a long generation time. It can be used for assaying antibiotics in serum, meast, foodstuffs etc.
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