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Process for preparing stabilised erythrocyte prepns. (I), wherein blood (e.g. from rabbit, rat, dove, sheep, chicken, man, etc.) is mixed with an isotonic anti-
Process for preparing stabilised erythrocyte prepns. (I), wherein blood (e.g. from rabbit, rat, dove, sheep, chicken, man, etc.) is mixed with an isotonic anti-
Process for preparing stabilised erythrocyte prepns. (I), wherein blood (e.g. from rabbit, rat, dove, sheep, chicken, man, etc.) is mixed with an isotonic anti-coagulant, centrifuged, and the erythrocytes washed with neutral buffer. They are then suspended in neutral buffer (10 vols.), treated with propan-1-al-2-one (1 vol.) overnight, washed, resuspended, treated with formalin (1 vol) and again washed. The erythrocytes may then be stored for a long time below 4 deg. without agglutinating or haemolysing spontaneously. This treatment has no noticeable effect on the normal immunological properties of the erythrocytes. - The erythrocytes may also be activated by treating with suitable antigens, pref. 1-24 hrs. at 24 deg. and pH3.6-7-0. Both activation and stability are improved by cooling to - 190 deg., and storing subsequently at -20 deg. for 16 weeks, by cycling for 6 weeks from -20 deg. (6-7 days) to +24 deg. (a few hrs.), or by treatment with NaIO4. Alternatively, activation may be carried out by fibrinogen treatment. The products can be stored at -190 deg. or freeze dried, until required. - Haemagglutination tests for detection and determination of gamma-globulins, bacterial and virus infections, histocompatability, auto immune diseases, and for assay of antigens.
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