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Extraction of a new polysaccharide substance from crude fucoidin
Extraction of a new polysaccharide substance from crude fucoidin
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机译：从岩藻依聚糖粗品中提取一种新的多糖物质
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摘要
The invention comprises a polysaccharide comprising a fucosemethylpentose ether sulphate polymer having a molecular weight of about 5,000 to about 50,000, a specific rotation of [a ]25D = -119,6 DEG in water, having in aqueous solution of 0,8% strength an ultra-violet absorption of very weak character in the range 256-280mm and being devoid of additional absorption maxima at 220-600 mm , having in 0,85 saline solution a relative viscosity of 1,2 being amorphous in character, containing the elements carbon, hydrogen, oxygen, sulphur, sodium, potassium and calcium, and being soluble in water. The novel polysaccharide is obtained by fractional precipitation of an aqueous solution of crude fucoidin by means of a water-miscible organic solvent and collecting the polysaccharide precipitated as the concentration of the solvent in the solution is increased from about 45% to about 67%, or by treating the said aqueous solution with a quaternary ammonium compound, preferably one containing a carbon chain of 8 carbon atoms or more, collecting the water-insoluble precipitate, breaking up the obtained quaternary ammonium-polysaccharide complex by the addition of a strongly ionizable salt and collecting the polysaccharide from the obtained solution with a water-miscible organic solvent. Both the fractional precipitation method and the quaternary ammonium complex formation may be used. The new polysaccharide may be further purified by dialysis. The product possesses anticoagulant and antilipemic activity. An example also describes the extraction of crude fucoidin from ground, air-dried Fucus vesiculosus by heating with water, adding toluene, diluting and filtering the supernatant fluid, adding lead acetate solution and filtering, adding barium hydroxide to the filtrate, shaking the resultant precipitate with sulphuric acid, and dialysing for 3 days. The non-dialysable material is concentrated in vacuo, freed of insoluble material by spinning at 12,000 g. for 45 minutes, the supernatant is poured into ethanol and dried in vacuo over P2O5. The material is designated as crude fucoidin.ALSO:A new polysaccharide comprising a fucosemethylpentose ether sulphate polymer (obtained from crude fucoidin, see Group IV (a)) having anticoagulant and antilipemic properties, may be dispersed in any of the usual dosage unit forms of pharmaceutical preparations, for example, tablets, pills, capsules, solutions, powders, with any desired excipients, binders, fillers or other therapeutically inert ingredients. For parenteral administration, the polysaccharide may be dissolved in any suitable liquid medium which is compatible with body fluids, e.g. physiological saline solution. For oral administration, the polysaccharide may be employed with or without other physiological active ingredients in tablets, pills or capsules.
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机译：本发明包括多糖，其包含岩藻糖甲基戊糖醚硫酸盐聚合物，其分子量为约5,000至约50,000，在水中的比旋光度[a] 25 D = -119,6°，在水溶液中具有0.8％的强度，紫外线吸收，在256-280mm范围内具有非常弱的特性，并且在220-600mm处没有附加的吸收最大值，在0.85的盐水溶液中相对粘度为1,2，为非晶态，含有碳元素，氢，氧，硫，钠，钾和钙，可溶于水。通过用水可混溶的有机溶剂分步沉淀粗制岩藻毒素的水溶液并收集随着溶液中溶剂浓度从约45％增至约67％而沉淀的多糖来获得新型多糖通过用季铵化合物，优选含有8个碳原子以上的碳链的季铵化合物处理所述水溶液，收集水不溶性沉淀物，通过加入强电离性盐将所得的季铵-多糖复合物分解，并与水混溶的有机溶剂从获得的溶液中收集多糖。分步沉淀法和季铵络合物的形成都可以使用。新的多糖可以通过透析进一步纯化。该产品具有抗凝血和抗血脂活性。一个例子还描述了通过用水加热，添加甲苯，稀释和过滤上清液，从醋酸铅溶液中过滤，向滤液中添加氢氧化钡，摇动所得沉淀物，从地面，风干的墨角藻中提取岩藻素的粗品。用硫酸，并透析3天。将不可透析的材料在真空中浓缩，通过以12,000g旋转除去不溶的材料。保持45分钟，将上清液倒入乙醇中并在P 2 O 5上真空干燥。该物质被称为粗岩藻糖苷。ALSO：含有岩藻糖甲基戊糖醚硫酸盐聚合物的新多糖（得自粗岩藻糖苷，见第IV（a）组），具有抗凝血和抗血脂特性，可以以下列任何常规剂量单位形式分散：药物制剂，例如片剂，丸剂，胶囊剂，溶液剂，散剂，以及任何所需的赋形剂，粘合剂，填充剂或其他治疗惰性成分。对于肠胃外给药，可以将多糖溶解在与体液相容的任何合适的液体介质中，例如，油。生理盐水溶液。对于口服给药，可以在片剂，丸剂或胶囊剂中与或不与其他生理活性成分一起使用多糖。
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