A method for preparing a ratiometric fluorescent baicalin probe based on a silver nanocluster AgNCs/black phosphorus quantum dot BPQDs double-doped metal organic framework MOF complex. Blue fluorescent BPQDs are reacted with a MOF precursor to prepare a BPQDs/MOF complex, red fluorescent AgNCs are added and the reaction is continued, BPQDs are encapsulated in MOF, and the AgNCs are adsorbed in MOF pores. Baicalin is added into an aqueous dispersion of an AgNCs/BPQDs/MOF complex containing catalase, and the baicalin generates H2O2 under the action of catalase. H2O2 causes fluorescence quenching of AgNCs, but has little effect on the fluorescence of BPQDs encapsulated in MOF. A linear relationship between a fluorescent emission peak intensity ratio IBPQDs/IAgNCs and the concentration of baicalin is fitted to construct a ratiometric fluorescent probe for efficient detection of baicalin.
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机译：一种基于银纳米光栅AGNCs /黑磷量子点BPQDS双掺杂金属有机框架MOF复合物的比率荧光黄芩苷探针制备比例荧光黄芩苷探针的方法。蓝荧光BPQD与MOF前体反应以制备BPQDS / MOF复合物，加入红色荧光AGNC，并继续反应，将BPQD包封在MOF中，并且AgNC被吸附在MOF孔中。将黄芩苷加入到含有过氧化氢酶的AgNCs / BPQDS / MOF复合物的水分散体中，并且在过滤酶的作用下，黄芩苷产生H 2 o 2 。 H 2 O 2 导致AGNC的荧光猝灭，但对封装在MOF中的BPQDS的荧光几乎没有影响。荧光发射峰强度比I BPQDS I 与黄芩苷浓度之间的线性关系，以构建比例荧光探针，以便有效地检测Baicalin。
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