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Strain of bacteria producing DHA and EPA, six gene fragments in the bacterial genome and their applications
Strain of bacteria producing DHA and EPA, six gene fragments in the bacterial genome and their applications
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机译：产生DHA和EPA的细菌菌株，细菌基因组中的六个基因片段及其应用
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摘要
The present invention discloses a strain of bacteria producing DHA and/or EPA, six gene fragments in the bacterial genome, and uses thereof. The strain is Schizoochytrium limacinum HS01, which has the accession number of CGMCC No. 13746 at China General Microbiological Culture Collection Center. The six gene fragments are composed of gene fragment 1 to gene fragment 6, and the nucleotide sequences are sequentially as shown in SEQ ID NO: 3 to SEQ ID NO: 8 in the Sequence Listing. The experiments prove that fermentation broth containing DHA and EPA can be obtained by fermenting Schizoochytrium limacinum HS01; the recombinant strain is obtained by introducing gene fragment 1 to gene fragment 6 into Schizoochytrium limacinum MYA-1381; the ability the recombinant strain for producing DHA and EPA is greatly improved. The bacteria provided by the invention, the six gene fragments, the protein encoded by these six gene fragments, the vector, the cell or the organism containing these six gene fragments all have important application values.
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机译：本发明公开了产生DHA和/或EPA的细菌菌株，细菌基因组中的六个基因片段，以及其用途。该菌株是 Schizoochytrium Limacinum HS01，其在中国一般微生物培养中心的CGMCC号13746号登记号。六个基因片段由基因片段1与基因片段6组成，并且核苷酸序列在序列列表中以SEQ ID NO：3至SEQ ID NO：8所示。实验证明，含有DHA和EPA的发酵液可以通过发酵 Schizoochytrium Limacinum HS01获得;通过将基因片段1引入基因片段6中的重组菌株，进入 Schizoochytrium Limacinum Mya-1381;能够大大提高了生产DHA和EPA的重组菌株。本发明提供的细菌，六个基因片段，由这六个基因片段，载体，细胞或含有这六个基因片段的含有这六个基因片段的生物体编码的蛋白质都具有重要的应用值。
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