首页>
外国专利>
Overexpression of N-glycosylation pathway regulators to regulate glycosylation of recombinant proteins
Overexpression of N-glycosylation pathway regulators to regulate glycosylation of recombinant proteins
展开▼
机译：N-糖基化途径调节剂的过度表达调节重组蛋白的糖基化
展开▼
页面导航
摘要
著录项
相似文献
摘要
PROBLEM TO BE SOLVED: To provide a method for adjusting the characteristics of a cell culture expressing a protein of interest. A method of controlling a high mannose-type glycoform content of a recombinant protein during a mammalian cell culture process, in which a host cell is transfected to overexpress a protein involved in the N-glycosylation pathway. The proteins involved in the N-glycosylation pathway include N-acetyl-glucosamine transferase-1 (encoded by Mgat1); N-acetyl-glucosamine transferase-2 (encoded by Mgat2); A method selected from the group consisting of UDP-galactose transporters encoded by Slc35a2; and combinations thereof. [Selection diagram] None
展开▼
