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AMPLIFICATION OF PAIRED PROTEIN-CODING MRNA SEQUENCES
AMPLIFICATION OF PAIRED PROTEIN-CODING MRNA SEQUENCES
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机译:配对蛋白质编码mRNA序列的扩增
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摘要
The present disclosure generally relates to sequencing two or more genes expressed in a single cell in a high-throughput manner using reverse transcriptases. More particularly, the present disclosure relates to a method for high-throughput sequencing of pairs of transcripts co-expressed in single cells (e.g., antibody VH and VL coding sequence) to determine pairs of polypeptide chains that comprise immune receptors.
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