PROBLEM TO BE SOLVED: To measure the concentration of metabolites and redox substances in cultured cells, and to measure the intracellular concentration by the fluorescence method, but it is difficult to measure the in vivo redox potential. SOLUTION: A temperature is set to 37 ° C. (310K) in an equation for giving a redox potential E according to the Nernst equation, and the redox potential E of hydrogen / hydrogen ions is E = -0.061 × pH + 0.031 × pH 2 (V). It becomes. At pH = 7.4, E = -0.451 + 0.031 x pH 2 (V). Measure the hydrogen gas index pH 2 of the subject and calculate E using the above formula. According to the potential verification method of the present invention, by blowing air containing air and hydrogen gas into the phosphate buffer solution, the potential before and after the blowing is measured, necessary corrections are made, and a linear regression line is obtained. , It is possible to confirm the change in potential with the change in hydrogen gas index pH 2 , and it can be seen that the redox potential obtained by calculation is close to the actual redox potential. [Selection diagram] Fig. 1
展开▼
