MUTANT REVERSE TRANSCRIPTASE WITH INCREASED THERMAL STABILITY AS WELL AS PRODUCTS, METHODS AND USES INVOLVING THE SAME

• 1. 具有热稳定性增加的突变型逆转录酶以及涉及其的产物、方法和用途 [P] . 中国专利： CN110709513A . 2020-01-17
• 2. 具有增加的稳定性的突变型乳酸氧化酶以及涉及其的产品、方法和用途 [P] . 中国专利： CN103013940B . 2015.03.25
• 3. MUTANT REVERSE TRANSCRIPTASE WITH INCREASED THERMAL STABILITY AS WELL AS PRODUCTS, METHODS AND USES INVOLVING THE SAME [P] . 欧洲知识产权局专利： EP3610006A1 . 2020-02-19

  机译： 具有增加的热稳定性的突变型逆转录酶以及涉及该酶的产品，方法和用途

• 4. MUTANT REVERSE TRANSCRIPTASE WITH INCREASED THERMAL STABILITY AS WELL AS PRODUCTS, METHODS AND USES INVOLVING THE SAME [P] . 美国专利： US2020040316A1 . 2020-02-06

  机译： 具有增加的热稳定性的突变型逆转录酶以及涉及该酶的产品，方法和用途

• 5. MUTANT REVERSE TRANSCRIPTASE WITH INCREASED THERMAL STABILITY AS WELL AS PRODUCTS, METHODS AND USES INVOLVING THE SAME [P] . 世界知识产权组织专利： WO2018189184A1 . 2018-10-18

  机译： 具有增加的热稳定性的突变型逆转录酶，以及涉及其的产品，方法和用途

• 1. Inhibition of telomerase with human telomerase reverse transcriptase antisense increases the sensitivity of tumor necrosis factor-α-induced apoptosis in prostate cancer cells [J] . Xiao-Dong Gao, Yi-Rong Chen 亚洲男性学杂志：英文版 . 2007,第005期
• 2. SYBR Green I-based product-enhanced reverse transcriptase assay for quantification of retroviral PFV and detection of the divalent cation preference of PFV RT [J] . Xiaofang, Yi, Yan, 中国病毒学：英文版 . 2014,第005期
• 3. Development of Reverse Transcriptase Loop-Mediated Isothermal Amplification for Rapid and Visualized Detection of Classical Swine Fever Virus [J] . QU Guang-gang, YANG Hui, TANG Na, 动物与饲料科学：英文版 . 2010,第003期
• 4. EFFECTIVE METHODS TO INCREASE THERMALSTABILITY OF SHALLOW EMITTER SOLAR CELLS WITH ALUMINIUM FRONT CONTACTS [C] . Mihail Manole, Rüdiger Meyer, Peter Engelhart, European photovoltaic solar energy conference . 2005
• 5. Stability Indicating Method Development and Validation for the Determination of Felodipine Raw Material and Degradant Products by Utilizing Reversed-phase Liquid Chromatography [D] . Patel, Kanan 2018
• 6. Rapid Genome Detection of Schmallenberg Virus and Bovine Viral Diarrhea Virus by Use of Isothermal Amplification Methods and High-Speed Real-Time Reverse Transcriptase PCR [O] . Andrea Aebischer, Kerstin Wernike, Bernd Hoffmann, 2014
• 7. Increased thermostability and fidelity of DNA synthesis of wild-type and mutant HIV-1 group O reverse transcriptases. [O] . Álvarez, Mar, Matamoros Grande, Tania, Menéndez-Arias, Luis 2009

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. SYBR Green Ⅰ-based product-enhanced reverse transcriptase assay for quantification of retroviral PFV and detection of the divalent cation preference of PFV RT [J] . Xiaofang Yi ,Yan Sun ,Qingmei Liu . 中国病毒学 . 2014,第005期
• 3. 利用计算机配置方法保护涉及计算机程序的实体装置产品 [C] . 张欣 . 2010年中华全国专利代理人协会年会暨首届知识产权论坛 . 2010
• 4. 基于立体视觉的具有相同特征的多目标匹配方法研究 [A] . 严念 . 2014