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NEW ENZYME-FREE GLUCOSE SENSOR APPLIED TO IN-SITU TEST IN FERMENTATION PROCESS
NEW ENZYME-FREE GLUCOSE SENSOR APPLIED TO IN-SITU TEST IN FERMENTATION PROCESS
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机译：新的无酶葡萄糖传感器应用于发酵过程中原位试验
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摘要
Disclosed is a new enzyme-free glucose sensor applied to an in-situ test in a fermentation process. The sensor can test the concentration of glucose in a fermentation liquor. The present invention comprises the following content: preparing an Au@Ni/C nano-composite with a core-shell structure by using a high-temperature oleylamine reduction method, and according to this, preparing a working electrode; constructing a three-electrode test system; three electrodes capturing an electrical signal generated by the working electrode performing electrocatalytic oxidation on glucose; obtaining data corresponding to the concentration of the glucose; and carrying out calculation to obtain the concentration of the glucose. Compared with existing enzyme glucose biosensors, the sensor in the present invention does not need complex immobilization technology, is resistant to high temperatures, can be used for an in-situ test, is high in precision, has catalytic activity not influenced by fermentation environments such as temperature and humidity, has good stability, and can be applied to a long-term fermentation process on-line test environment. Compared with existing enzyme-free glucose biosensors, the sensor in the present invention works under a relatively low working voltage, is high in selectivity, is not influenced by adsorption of a large number of chloride ions present in a fermentation liquor, and is highly anti-toxic. Therefore, the sensor becomes an enzyme-free electrochemical sensor applied to an in-situ test of glucose in a fermentation process.
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机译：公开了一种在发酵过程中施加到原位试验的新酶无血糖传感器。传感器可以在发酵液中测试葡萄糖的浓度。本发明包括以下内容：通过使用高温油胺还原方法，用核心 - 壳结构制备Au @ Ni / C纳米复合物，并根据这，制备工作电极;构建三电极测试系统;三个电极捕获由在葡萄糖上进行电催化氧化的工作电极产生的电信号;获得对应于葡萄糖浓度的数据;并进行计算以获得葡萄糖的浓度。与现有的酶葡萄糖生物传感器相比，本发明中的传感器不需要复杂的固定化技术，耐高温，可用于原位试验，精确度高，具有催化活性不受发酵环境的影响作为温度和湿度，具有良好的稳定性，并且可以应用于长期发酵过程在线测试环境。与现有的无酶葡萄糖生物传感器相比，本发明中的传感器在相对较低的工作电压下工作，选择性高，不受发酵液中存在的大量氯离子的吸附，并且具有高度抗-有毒的。因此，传感器成为一种酶的电化学传感器，其应用于发酵过程中葡萄糖的原位试验。
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