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VIABLE TISSUE FORMS AND METHODS FOR MAKING AND USING SAME

机译:可行的组织形式和制造和使用方法

摘要

Preserved tissue samples contain endogenous viable cells and retain or promote biological activity after being stored at temperatures above freezing for extended periods of time (e.g., from 14 days to 3 years). The preserved tissue samples are implanted in or on a subject and, after rehydration, they retain beneficial biological activity, promote beneficial biological activity, or both. The beneficial biological activity comprises promoting one or more of tissue healing, tissue growth, and tissue generation. Methods for preparing the preserved tissue samples include contacting a recovered tissue sample with one or more protectants, followed by lyopreservation. Suitable protectants include sugars, polyphenols, carotenoids, and combinations thereof. Preferred protectants include glucose, fructose, sucrose, trehalose, dextran, EGCG, and combinations thereof. The recovered tissue sample may be any of several possible issue types. In preferred embodiments, the recovered tissue samples are selected from bone, placental, cartilage and combinations thereof.
机译:保存的组织样品含有内源性活细胞并在储存在冻结的温度后保持或促进生物活性,延长时间(例如,从14天至3年中)。保存的组织样品在受试者中或在受试者中植入,然后再水合后,它们保留有益的生物活性,促进有益的生物活性,或两者。有益的生物活性包括促进组织愈合,组织生长和组织产生中的一种或多种。制备保存的组织样品的方法包括使回收的组织样品与一种或多种保护剂接触,然后是氯置。合适的保护剂包括糖,多酚,类胡萝卜素及其组合。优选的保护剂包括葡萄糖,果糖,蔗糖,海藻糖,葡聚糖,EGCG及其组合。回收的组织样本可以是几种可能的问题中的任何一种。在优选的实施方案中,回收的组织样品选自骨,胎盘,软骨及其组合。

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