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PRIMERS FOR DETECTING TRACE AMOUNT OF RARE SINGLE-NUCLEOTIDE VARIANT AND METHOD FOR SPECIFICALLY AND SENSITIVELY DETECTING TRACE AMOUNT OF RARE SINGLE-NUCLEOTIDE VARIANT BY USING SAME
PRIMERS FOR DETECTING TRACE AMOUNT OF RARE SINGLE-NUCLEOTIDE VARIANT AND METHOD FOR SPECIFICALLY AND SENSITIVELY DETECTING TRACE AMOUNT OF RARE SINGLE-NUCLEOTIDE VARIANT BY USING SAME
The present invention relates to a primer set for detecting a trace amount of a rare single-nucleotide variant and a method for specifically and sensitively detecting a trace amount of a rare single-nucleotide variant by using same. Particularly, the present invention relates to: a primer composition for detecting a single-nucleotide variant (SNV), the primer composition comprising a first primer that comprises a nucleotide sequence complementary to a to-be-detected DNA fragment comprising an SNV to be detected, and at the 3' end of the primer, 2-6 nucleotide sequences that are not complementary to the to-be-detected DNA fragment; and a method for detecting a trace amount of a rare single-nucleotide variant, the method comprising performing a polymerase chain reaction (PCR) using the composition.
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