Method for increasing seed germination rate and embryo swelling rate of Bletilla striata

• 1. 一种盐碱地原位提高植物种子萌发率及幼苗成活率的方法 [P] . 中国专利： CN113068590A . 2021-07-06
• 2. 一种提高合柱金莲木种子萌发率及幼苗成活率的方法 [P] . 中国专利： CN112470904A . 2021-03-12
• 3. MANUFACTURING METHOD OF SPROUTED EMBRYO WITH INCREASED BIOACTIVE SUBSTANCES AND IMPROVED NUTRITIONAL FUNCTIONS OF THE EMBRYO SEPARATED FROM SOY SEEDS [P] . 韩国专利： KR20130057173A . 2013-05-31

  机译： 从大豆种子中分离出具有增加的生物活性物质和改善的营养功能的芽状胚的制造方法

• 4. APPARATUS FOR IMPROVING GERMINATION RATE OF SEEDS UTILIZING HIGH VOLTAGE AND METHOD OF IMPROVING GERMINATION RATE OF SEEDS USING THE SAME [P] . 日本专利： JP2001136812A . 2001-05-22

  机译： 利用高压提高种子发芽率的装置和使用相同方法提高种子发芽率的方法

• 5. METHOD FOR TREATING LETTUCE SEEDS FOR INCREASING SEED GERMINATION RATE [P] . 韩国专利： KR20160029512A . 2016-03-15

  机译： 处理生菜种子以提高种子发芽率的方法

• 1. Effects of Different Processing Methods on Germination Rate of Wada Alfalfa Seeds [J] . LI Xiao-an 动物与饲料科学：英文版 . 2011,第004期
• 2. A Vision Based Method for Automatic Evaluation of Germination Rate of Rice Seeds [C] . Thuy Thi Nguyen, Van-Nam Hoang, Thi-Lan Le, 2018 1st International Conference on Multimedia Analysis and Pattern Recognition . 2018
• 3. Analytical and Computational Tools to Assess Seed Quality and Model Germination Rates [D] . Bello, Pedro Henrique Nogueira. 2020
• 4. A METHOD OF INCREASING THE RATE OF SEED GERMINATION OF TARAXACUM KOK-SAGHYZ [O] . J. Levitt, P. C. Hamm 1943
• 5. Embryo Elongation and Germination Rates as Sensitive Indicators of Lettuce Seed Quality: Priming and Aging Studies [O] . G. Hacisalihoglu, D.H. Paine, M.B. Hilderbrand, 1999

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Teaching an Old Dog a New Trick: Multifaceted Strategies to Control Primary Seed Germination by DELAY OF GERMINATION 1 (DOG1) [J] . Ping Li ,Huanhuan Ni ,Songbei Ying . 国际实验植物学杂志(英文) . 2020,第1期
• 3. Effect of Increased Nitrogen Application Rates and Environment on Protein, Oil, Fatty Acids, and Minerals in Sesame (iSesamum indicum/i) Seed Grown under Mississippi Delta Conditions [J] . Nacer Bellaloui ,Hamed K. Abbas ,M. Wayne Ebelhar . 食品与营养科学（英文） . 2018,第9期
• 4. Impacts of snow on seed germination are independent of seed traits and plant ecological characteristics in a temperate desert of Central Asia [J] . Anlifeire ANNIWAER ,SU Yangui ,ZHOU Xiaobing . 干旱区科学：英文版 . 2020,第5期
• 5. 提高锁阳种子萌发率的研究 [C] . 刘睿杰 ,苏格尔 ,尹诚国 . 中药材规范化种植研究研讨会 . 2004
• 6. Ⅰ胚胎干细胞向肝细胞诱导分化的体外实验研究;Ⅱ提高胚胎干细胞向肝细胞诱导分化率的体外实验研究;Ⅲ胚胎干细胞向胆管上皮细胞诱导分化的体外实验研究 [A] . 胡安斌 . 2006