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A YEAST PHENOTYPIC SCREENING METHOD FOR ISOLATION OF FUNCTIONAL ANTIBODIES AGAINST G-PROTEIN COUPLED RECEPTORS
A YEAST PHENOTYPIC SCREENING METHOD FOR ISOLATION OF FUNCTIONAL ANTIBODIES AGAINST G-PROTEIN COUPLED RECEPTORS
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机译：一种酵母表型筛选方法，用于将函数抗体分离对G蛋白偶联受体的影响
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摘要
The present invention provides a method for identifying a functional antibody or antigen-binding protein or a fragment thereof that is capable of binding to, and stimulating the activity of, a target transmembrane protein comprising the steps of providing yeast cells transformed with yeast expression vectors encoding a library of antibodies or antigen-binding proteins or fragments thereof, wherein said yeast cells express said target transmembrane protein, expressing said library of antibodies or antigen-binding proteins or fragments thereof in the yeast cells, wherein said expressed antibodies or antigen-binding proteins or fragments thereof are secreted into the periplasmic space of said yeast cells, incubating the yeast cells in or on a selective medium or under a restrictive temperature, or a combination thereof, and detecting a predetermined phenotype in the yeast cells, wherein detection or manifestation of the predetermined phenotype is indicative of binding of the functional antibody or antigen-binding protein or a fragment thereof to said target transmembrane protein and stimulation of said target transmembrane protein by the functional antibody or antigen-binding protein or a fragment thereof. Antibodies or antibody fragments that are agonists of G-protein coupled receptors identified by the method of the invention are also provided.
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