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Identification and / or identification method of Bacillus cereus species and related species using MALDI-TOF MS

机译:使用MALDI-TOF MS的芽孢杆菌物种和相关物种的鉴定和/或鉴定方法

摘要

To provide methods for discriminating and/or identifying Bacillus cereus species and close species thereof rapidly and accurately, and to provide biomarker sets used for the same.SOLUTION: Using as a biomarker at least one of ribosomal proteins L33, L30, L29, S18, L31, S20 and L22 of Bacillus cereus species and close species thereof, these species are discriminated rapidly and accurately by (a) analyzing by MALDI-TOF MS method to obtain mass spectrum including analysis values as regards a biomarker, and by (b) comparing the analysis value of the biomarker with the known analysis value of the biomarker previously obtained from Bacillus cereus species and close species thereof to discriminate between the species which gives a known analysis value different from the analysis value and the species of analysis subject. Identification of these species can also be performed using this discrimination method.SELECTED DRAWING: None
机译:提供用于迅速和准确地识别和/或鉴定杆菌种类和密封芽孢杆菌物种的方法,并提供用于相同的生物标志物组。溶液:用作生物标志物核糖体蛋白L33,L30,L29,S18中的至少一种。 芽孢杆菌种类的L31,S20和L22及其接近物种,通过MALDI-TOF MS方法进行快速且准确地通过(a)来歧视,以获得包括分析值的质谱,并通过(b)比较 生物标志物的分析值与先前从芽孢杆菌物种获得的生物标志物的已知分析值,接近物种以区分物种,其给出与分析值不同的已知分析值和分析对象的种类。 也可以使用这种辨别方法进行这些物种的识别。选择绘图:无

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