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METHOD FOR CULTURING COLORECTAL CANCER SOLID TUMOR PRIMARY CELLS AND COLORECTAL CANCER ASCITES PRIMARY TUMOR CELLS AND SUPPORTING REAGENTS
METHOD FOR CULTURING COLORECTAL CANCER SOLID TUMOR PRIMARY CELLS AND COLORECTAL CANCER ASCITES PRIMARY TUMOR CELLS AND SUPPORTING REAGENTS
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机译：培养结直肠癌固体肿瘤原发性细胞和结直肠癌腹水原发性肿瘤细胞的方法和支持试剂
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摘要
A method for culturing colorectal cancer solid tumor primary cells and colorectal cancer ascites primary tumor cells and supporting reagents. A method for culturing colorectal cancer solid tumor primary cells and colorectal cancer ascites primary tumor cells and supporting reagents. Colorectal cancer solid tumor tissues are treated with mild cell dissociation reagents, and colorectal cancer cells are isolated from ascites with a mild method, thereby ensuring the vitality of cancer cells to the greatest extent. A special serum-free medium is prepared, and colorectal cancer solid tumor-derived tumor cells are cultured in vitro with a suspension culture system to ensure normal expansion of the cancer cells while eliminating the interference of normal cells to the greatest extent. The colorectal cancer primary cell culture obtained by the method are usable for in vitro experiments, second-generation sequencing, building of animal models, and building of cell lines at multiple cell levels.
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