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Liquid composition for Microinsemination, unfertilized egg, fertilized egg and liquid composition for embryo activation, micro insemination method using the same, and method for activating unfertilized egg, fertilized egg and embryo
Liquid composition for Microinsemination, unfertilized egg, fertilized egg and liquid composition for embryo activation, micro insemination method using the same, and method for activating unfertilized egg, fertilized egg and embryo
Problem to be solved: to provide a liquid composition for Microinsemination, an unfertilized egg, a fertilized egg, a liquid composition for embryo activation, a micro insemination method using the same, an activation method of an unfertilized egg, a fertilized egg, and an embryo, which can be carried out in a microscopic manner.Solution: sodium ion of 0 to 35 mmol / LPotassium ion of 30 to 50 mmol / L5 to 10 mmol / L of magnesium ionsCalcium ion of 0.5 to 2 mmol / L15 to 35 mmol / L phosphoric acid0 to 2 mmol / L chloride ions and5 to 15 mmol / L carbonate ionsA liquid composition for microscopic fertilization comprising 5-10 mmol / L sulfate ionsFurthermore, unfertilized egg containing pyruvic acid or its salt and lactate or salt thereofFertilized egg and liquid composition for embryo activationMicroscopic fertilization method and unfertilized egg using themMethod of activating fertilized egg and embryo.Diagram
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