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THREE-DIMENSIONAL CULTURE METHOD, THREE-DIMENSIONAL CULTURE STRUCTURE, AND PRODUCTION METHOD FOR THREE-DIMENSIONAL CULTURE STRUCTURE

机译：三维培养方法，三维培养结构和三维培养结构的生产方法

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摘要

This three-dimensional culture method comprises: a step for preparing a cell suspension that contains cells (12C) and a culture medium (14M); a step for preparing a solid surface (10S) formed from a porous alumina layer that has a plurality of first recesses; a step for adhering droplets (16D) of the cell suspension on the solid surface; and a step for culturing the cells in the droplets while in a state in which the solid surface faces the direction in which gravity acts on the droplets.

机译：该三维培养方法包括：制备含有细胞（12c）和培养基（14m）的细胞悬浮液的步骤; 制备由具有多个第一凹槽的多孔氧化铝层形成的固体表面（10s）的步骤; 用于将细胞悬浮液的液滴（16d）粘附在固体表面上的步骤; 并且在固体表面面向重力作用在液滴上的状态下培养液滴中的细胞的步骤。

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公开/公告号WO2021182307A1

专利类型
公开/公告日2021-09-16

原文格式PDF
申请/专利权人 SHARP KABUSHIKI KAISHA;TOTTORI INSTITUTE OF INDUSTRIAL TECHNOLOGY;
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申请/专利号WO2021JP08513
发明设计人 TAGUCHI TOKIO;TERANISHI TOMOKO;NAKAMATSU KENICHIRO;YAMANE YU;SHIBAI YASUHIRO;TAKEBAYASHI ISAO;SUGINE MITSUAKI;SUGIMOTO YUKO;
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申请日2021-03-04
分类号C12N5/071;C12M3;
国家 JP
入库时间 2022-08-24 21:07:50

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